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Prof. Awtar Krishan

Affiliation : Emeritus Professor, University of Miami-School of Medicine, USA and Co-Chair of the Live Education Task Force of The International Society for Advancement of Cytometry (ISAC)

Title of the Talk/Lab :History of INDO-US Flow Cytometry Workshops

Awtar Krishan obtained PhD in Zoology (cytogenetics) from the Panjab University, Chandigarh, India (1962) and a PhD in Micro-Anatomy from the Univ. of Western Ontario, Canada (1963).  From 1965 to 1977, he was at the Sidney Farber Cancer Institute, Harvard Medical School, Boston, USA as Head of the Tissue Ultrastructure and Experimental Pathology Labs. He transferred to the University of Miami-School of Medicine and from 1980 to 1993 was Scientific Director of the Comprehensive Cancer Center.  Most of his work has focused on avian cytogenetics, experimental therapeutics and analytical cytology.

He developed flow cytometric techniques for rapid analysis of DNA content by the propidium iodide staining method (1), monitoring of fluorescent drug retention and resistance and for hormone receptor and marker expression in tumor cells (2-4).

Dr. Krishan has organized cytometry workshops in India, Thailand, China, Egypt, Turkey, Malaysia, and Singapore. These activities are described in the website: http://www.cytometryworkshops.com. He is Co-Chair of the Live Education Task Force of The International Society for Advancement of Cytometry (ISAC).

Relevant Literature:

  1. Krishan A. Rapid flow cytofluorometry analysis of mammalian cell cycle by propidium iodide staining. J Cell Biol 66:188- 193, 1975.
  2. Sharma S, Cabana R, Shariatmadar S, Krishan A. Cellular volume and marker expression in human peripheral blood apheresis stem cells. Cytometry A. 73A:160-167, 2008.
  3. Krishan A, Ganjei-Azar P, Hamelik R, Sharma D, Reis I, Nadji M. Flow immunocytochemistry of marker expression in cells from body cavity fluids.  Cytometry A. 77:132-43, 2010.   PMID: 19899128
  4. Krishan A, Sharma D, Sharma S, Hamelik RM, Ganjei-Azar P, Nadji M. ALDH(+)/CD44(+)/CD24(-) expression in cells from body cavity fluids. Cytometry B Clin Cytom. 78:176-82, 2010. PMID: 20043317.

 

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