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Prof. Gunnur Deniz

Affiliation : Director of Aziz Sancar Institute of Experimental Medicine and Head of Department of Immunology, Istanbul University, Turkey

Title of the Talk/Lab :Flow Cytometry and Characterization of NK Cell response during Infectious Diseases

Gunnur Deniz, graduated from Istanbul University, Faculty of Sciences, Department of Biology in 1985 and received her M.Sc. degree in Physiology in 1989. Between 1990-1994 she was granted by the Turkish Republic Higher Education Council (YÖK) with a PhD fellowship to study at the Department of Immunology, University of Liverpool and received her PhD degree in immunology in 1994. She is the chairman of the Immunology Department at Aziz Sancar Institute of Experimental Medicine (DETAE) since 2002, and has also been working as a President of Aziz sancar Institue of Experimental Medicine since 2016. She is the past president of the Turkish Society of Immunology. Most of her work is focused on functional and phenotypical characterization of NK cells.

Lecture: Flow Cytometry and Characterization of NK Cell response during Infectious Diseases: Natural killer (NK) cells are lymphocytes of the innate immune system that can recognize and kill various types of malignant cells and virus infected cells. NK cells were originally described as lymphocytes of the innate immune system capable of eliminating tumor and infected cells without prior antigen exposure. Flow cytometry is a rapid method for monitoring of NK cells, NK cell subsets and NK cytotoxic activity.  In this lecture, we will discuss flow cytometric methods for phenotypic detection and functional analysis of NK cells by flow cytometry.

Relevant Literature:

  1. Aktas E, Kucuksezer UC, Bilgic S, Erten G, Deniz G. Relationship between CD107a expression and cytotoxic activity. Cell Immunol. 2009;254(2):149-54.
  2. Deniz G, Akdis M. NK cell subsets and their role in allergy. Expert Opin Biol Ther. 2011;11(7):833-41.
  1. Deniz G, van de Veen W, Akdis M. Natural killer cells in patients with allergic diseases. J Allergy Clin Immunol. 2013;132(3):527-35.
  2. Kucuksezer UC, Zekiroglu E, Kasapoglu P, Adin Cinar S, Aktas Cetin E, Deniz G. A stimulatory role of ozone exposure on human natural killer cells. Immunol Invest. 2014;43(1):1-12.
  3. Kucuksezer UC, Aktas-Cetin E, Bilgic-Gazioglu S, Tugal-Tutkun I, Gül A, Deniz G. Natural killer cells dominate a Th-1 polarized response in Behçet’s disease patients with uveitis. Clin Exp Rheumatol. 2015;33(6 Suppl 94):S24-9.
  4. Ostapchuk YO, Aktas Cetin E, Perfilyeva YV, Yilmaz A, Skiba YA, Chirkin AP, Omarbaeva, AN,Talaeva SG, Belyaev NN, Deniz G. Peripheral blood NK cells expressing HLA-G, IL-10 and TGF-β in healthy donors and breast cancer Patients. Cell Immunol. 2015;298(1-2):37-46.
  5. Cosan F, Cetin Aktas E, Akdeniz N, Emrence Z, Cefle A, Deniz G. Natural killer cell subsets and their functional activity in Behçet’s Disease. Immunol Invest. 2017;46(4):419-432.
  6. Tahrali I, Kucuksezer UC, Altintas A, Uygunoglu U, Akdeniz N, Aktas-Cetin E, Deniz G. Dysfunction of CD3CD16+CD56dim and CD3CD16CD56brightNK cell subsets in RR-MS patients. Clin Immunol. 2018;193:88-97.
  7. 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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Eur J Immunol. 2019;49(10):1457-1973.
  8. Tahrali I, Kucuksezer UC, Akdeniz N, Altintas A, Uygunoglu U, Aktas-Cetin E, Deniz G. CD3CD56+NK cells display an inflammatory profile in RR-MS patients. Immunol Lett. 2019;(4)216:63-69.

 

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