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Prof. Jaroslav Doležel

Affiliation : Head, Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany & Professor, Molecular Biology and Genetics, Palacký University, Olomouc, Czech Republic

Title of the Talk/Lab :Role of Flow Cytometry in Plant Research

Jaroslav Doležel is Head of the Centre of Plant Structural and Functional Genomics of the Institute of Experimental Botany in Olomouc (Czech Republic), Research Director of the Centre of the Region Haná for Biotechnological and Agricultural Research in Olomouc, and Professor of Molecular Biology and Genetics at Palacký University in Olomouc. He obtained his PhD in genetics from Czechoslovak Academy of Sciences, Prague, Czechoslovakia, in 1983. He was a junior researcher at the Institute of Experimental Botany, Olomouc, from 1983 to 1985; from 1985 to 1986 he was a postdoctoral fellow at ENEA, Rome, Italy. Since 1986 he has been with the Institute of Experimental Botany (Olomouc).

His research focuses on plant genome organization, function and evolution. He developed methods for flow cytometric estimation of ploidy and genome size (1 – 4) and has pioneered flow cytometric chromosome sorting to facilitate genome sequencing and gene cloning in species with complex genomes (5 – 6). Dr. Dolezel published over 350 papers in scientific journals, which have been frequently cited, edited three books and was principal investigator and co-investigator in more than thirty national and international research projects. He is a member of the Learned Society of the Czech Republic and has received prestigious awards for his scientific achievements, including the National prize of the Czech government “Czech Mind” – the highest scientific award in the Czech Republic awarded each year to one scientist.

Dr. Doležel is frequently invited to give talks at international symposia, he served as FAO/IAEA expert in technical cooperation programs, and has been lecturing in many training courses in various countries. He is a co-founder of the Czech Society for Analytical Cytometry and served as its president from 2007 to 2011.

Lecture: Role of Flow Cytometry in Plant Research: In this lecture we will review the use of flow cytometry and sorting in plant biology with a special emphasis on the estimation of ploidy and genome size.

Relevant Literature:

  1. Doležel, J., Sgorbati, S., Lucretti, S.: Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. – Physiol. Plant. 85: 625-631, 1992.
  2. Doležel, J., Greilhuber, J., Lucretti, S., Meister, A., Lysák, M. A., Nardi, L., Obermayer, R.: Plant genome size estimation by flow cytometry: Inter-laboratory comparison. – Ann. Bot. 82 (Suppl. A): 17-26, 1998.
  3. Doležel, J., Greilhuber, J., Suda, J.: Estimation of nuclear DNA content in plants using flow cytometry. – Nat. Protoc. 2: 2233-2244, 2007.
  4. Greilhuber, J., Doležel, J., Lysák, M.A., Bennett, M.D.: The origin, evolution and proposed stabilization of the terms ‘genome size’, and ‘C-value’ to describe nuclear DNA contents. – Ann. Bot.-London 95: 255–260, 2005.
  5. Doležel, J., Vrána, J., Šafář, J., Bartoš, J., Kubaláková, M., Šimková, H.: Chromosomes in the flow to simplify genome analysis. – Funct. Integr. Genomics 12: 397-416, 2012.
  6. Zwyrtková, J., Šimková, H., Doležel, J.: Chromosome genomics uncovers plant genome organization and function. – Biotechnol. Adv. 46: 107659, 2021.

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